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生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1C23, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 133.8 kDa
observed mol wt ~17 kDa
純化經由
using Protein A
物種活性
human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
表位序列
C-terminal
Protein ID登錄號
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... JAG1(182)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 1C23 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Jagged 1. It targets an epitope within 24 amino acids from the C-terminal, cytoplasmic domain.
免疫原
KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal, cytoplasmic domain of human Jagged-1.
應用
Quality Control Testing
Evaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Jagged1 in human kidney tissue sections.
Tested applications
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Jagged1 in one million Jurkat cells.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Jagged1 in HT-29 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Jagged1 in HepG2 cells.
Affinity Binding Assay: A representative lot of this antibody bound Jagged1 with a KD of 1.1 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Jagged1 in human kidney tissue sections.
Tested applications
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Jagged1 in one million Jurkat cells.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Jagged1 in HT-29 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Jagged1 in HepG2 cells.
Affinity Binding Assay: A representative lot of this antibody bound Jagged1 with a KD of 1.1 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
標靶描述
Protein jagged-1 (UniProt: P78504; also known as Jagged1, hJ1, CD339) is encoded by the JAG1 (also known as JAGL1) gene (Gene ID: 182) in human. Jagged 1 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to generate the mature form that contains a large extracellular domain (aa 34-1067), a transmembrane domain (aa 1068-1093), and a cytoplasmic domain (aa 1094-1218). Jagged 1 is widely expressed in adult and fetal tissues. Its expression is observed in 32-52 days old embryos in the distal cardiac outflow tract and in pulmonary artery, pancreas, mesocardium, neural tube, and around the major bronchial branches. It serves as ligand for multiple Notch receptors and is involved in mediation of Notch signaling and cell-fate decisions during hematopoiesis. It is also reported to enhance FGF-induced angiogenesis. Its binding and activating ability are tightly regulated by Notch receptor glycosylation. Jagged 1 also plays roles in various aspects of cancer biology, including tumor angiogenesis, neoplastic cell growth, metastasis, epithelial-mesenchymal transition, and resistance to therapy. Its expression in other cells of tumor microenvironment has also been described. Jagged 1 expression can also be induced by other signaling pathways that are important in tumor growth and metastasis, including TGF-β, WNT/β-catenin, IL-6, and NF-kB. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, D., et al. (2014). Front. Oncol. 4; 254; Cordle, J., et al. (2008). Nat. Struct. Mol. Biol. 15(8); 849-857).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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