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Merck
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文件

T3913

Sigma-Aldrich

Tris-硼酸-EDTA缓冲溶液

5× concentrate, powder blend

别名:

TBE 缓冲液

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About This Item

MDL號碼:
MFCD00236358
分類程式碼代碼:
41105319
PubChem物質ID:
329826779
NACRES:
NA.25

形狀

powder blend

技術

electrophoresis: suitable

雜質

DNase, RNase and NICKase, none detected

pH值

8.1-8.5 (25 °C, 5 ×)

溶解度

water: 85.1 g/L, clear, colorless

適合性

suitable for gel electrophoresis (after dilution to working concentration)

應用

diagnostic assay manufacturing

SMILES 字串

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI 密鑰

OSBLTNPMIGYQGY-UHFFFAOYSA-N

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應用

Tris-硼酸-EDTA缓冲液适用于凝胶电泳。
它可用于以下研究：
  • 作为通过十二烷基硫酸钠-孔梯度电泳评估卵白蛋白、抗生物素蛋白和胎球蛋白糖蛋白分子量的缓冲系统。
  • 用于淀粉凝胶中的血红蛋白电泳。
  • 制备用于快速测定DNA序列的缓冲液浓度梯度凝胶。
TBE电泳液是DNA和RNA聚丙烯酰胺凝胶电泳最常用的缓冲液。TBE用于非变性或变性（7M 尿素）凝胶。 它通常也用于DNA自测序凝胶。 TBE可以用于琼脂糖凝胶，但是不推荐用于回收核酸的制备胶。
将TBE储存浓缩溶液稀释到1×TBE电泳缓冲液，生成的缓冲液含有89mM Tris-硼酸和2mM EDTA，pH8.3。 5X或10X储存液也可以加入丙烯酰胺/甲叉双丙烯酰胺储存液，用来制成PAGE胶。 为了达到最大的分辨率，推荐电压小于5V/cm(电极单元间距离）。

包裝

袋装

準備報告

采用经生物技术性能认证的 Trizma 碱（产品代码 T6066）、分子生物学试剂硼酸（产品代码 B6768）和 EDTA 二钠盐（产品代码 E5134）制备。

重構

溶解于指定量的水中得到 5× 浓缩液（0.445M Tris-硼酸、10mM EDTA，pH 为 8.3）。须提供合适的容器。

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H360FD

危險分類

Repr. 1B

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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THE AMINO ACID COMPOSITION OF HEMOGLOBIN. V. THE PREPARATION OF PURIFIED HEMOGLOBIN FRACTIONS BY CHROMATOGRAPHY ON CELLULOSE EXCHANGERS AND THEIR IDENTIFICATION BY STARCH GEL ELECTROPHORESIS USING TRIS-BORATE-EDTA BUFFER.
A I CHERNOFF et al.
Blood, 25, 646-661 (1965-05-01)
M D Biggin et al.
Proceedings of the National Academy of Sciences of the United States of America, 80(13), 3963-3965 (1983-07-01)
Two methods for increasing the length of DNA sequence data that can be read off a polyacrylamide gel are described. We have developed a rapid way to pour a buffer concentration gradient gel that, by altering the vertical band separation
Glycoprotein molecular-weight estimation using sodium dodecyl sulfate-pore gradient electrophoresis: comparison of tris-glycine and tris-borate-EDTA buffer systems.
J F Poduslo
Analytical biochemistry, 114(1), 131-139 (1981-06-01)
Xiang Li et al.
Nucleic acids research, 47(10), 5074-5085 (2019-06-05)
In microorganisms, a number of metalloproteins including PerR are found to regulate gene expression in response to environmental reactive oxygen species (ROS) changes. However, discovery of similar regulatory mechanisms remains elusive within mammalian cells. As an antioxidant metalloenzyme that maintains
Josep Balart et al.
Radiation oncology (London, England), 6, 6-6 (2011-01-18)
Radiation-induced DNA double-strand break (DSB) repair can be tested by using pulsed-field gel electrophoresis (PFGE) in agarose-encapsulated cells. However, previous studies have reported that this assay is impaired by the spontaneous DNA breakage in this medium. We investigated the mechanisms
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实验方案

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

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