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T3449

Sigma-Aldrich

TrypZean® 溶液 1×

recombinant, expressed in corn, sterile-filtered

别名:

胰蛋白酶

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.75

重組細胞

expressed in corn

品質等級

300

無菌

sterile-filtered

形狀

aqueous solution

濃度

1 ×

技術

cell culture | mammalian: suitable

pH值

7.70-8.30

運輸包裝

dry ice

儲存溫度

−20°C

相关类别

一般說明

TrypZean®溶液，1×是通过玉米中表达的重组牛胰蛋白酶TrypZean配制，并利用ProdiGene′专有的转基因植物蛋白表面系统生成。该产物经优化处理，适用于无血清和补充血清的贴壁细胞培养基中的细胞解离。

應用

TrypZean®溶液，1×已用于：

  • 在DNA提取过程中悬浮颗粒
  • 将细胞消化为单细胞悬液，用于2D细胞的药物分型
  • 重组胰蛋白酶，诱导IPT-AF培养基中继代培养的Vero细胞分裂

特點和優勢

TrypZean®溶液具有以下特点和优势：
  • 不含动物组分-消除病毒、BSE或其他潜在外来因子的风险
  • TrypZean是重组胰蛋白酶-使用相同的酶（具有相同的动力学）进行细胞分离意味着最少的实验方案更改
  • 酶抑制-大豆胰蛋白酶铁罐剂及其他抑制剂对TrypZean的作用与对天然胰蛋白酶的作用相同（以重量计）
  • 高纯度-TrypZean提供更高的特异性，消除低纯度酶中出现的污染活性
  • 方便快捷-TrypZean溶液是一种无菌过滤溶液，以最佳浓度配制而民，旨在解离贴壁细胞系

法律資訊

Trypzean is a registered trademark of ProdiGene, Inc.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

nwg

閃點（°F）

Not applicable

閃點（°C）

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
RNBL8259
RNBL4895
RNBL3434
RNBK7718
RNBK4189

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Samia Rourou et al.
Biotechnology progress, 25(6), 1752-1761 (2009-09-22)
This work describes the development of an animal-component free medium (IPT-AFM) that allows an optimal growth of Vero cells, an adherent cell line used for the production of viral vaccines. Statistical experimental design was applied to identify crucial nutrients that
Maria T Nelson et al.
Cell reports, 26(8), 2227-2240 (2019-02-21)
Metagenomic sequencing is a promising approach for identifying and characterizing organisms and their functional characteristics in complex, polymicrobial infections, such as airway infections in people with cystic fibrosis. These analyses are often hampered, however, by overwhelming quantities of human DNA, yielding
Katja Peschke et al.
EMBO molecular medicine, 14(4), e14876-e14876 (2022-02-05)
Despite the advance and success of precision oncology in gastrointestinal cancers, the frequency of molecular-informed therapy decisions in pancreatic ductal adenocarcinoma (PDAC) is currently neglectable. We present a longitudinal precision oncology platform based on functional model systems, including patient-derived organoids
Maria T Nelson et al.
Cell reports, 26(8), 2227-2240 (2019-02-21)
Metagenomic sequencing is a promising approach for identifying and characterizing organisms and their functional characteristics in complex, polymicrobial infections, such as airway infections in people with cystic fibrosis. These analyses are often hampered, however, by overwhelming quantities of human DNA, yielding
Clay O Lacefield et al.
Cell reports, 26(8), 2000-2008 (2019-02-21)
The mammalian brain can form associations between behaviorally relevant stimuli in an animal's environment. While such learning is thought to primarily involve high-order association cortex, even primary sensory areas receive long-range connections carrying information that could contribute to high-level representations.

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