T1075

Anti-Tal (CS-15) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 别名: Anti-Tsg101-associated ligase
• MDL號碼: MFCD06798551
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen 84 kDa
• 物種活性: rat, canine, human, mouse
• 技術: immunoprecipitation (IP): 5-10 μg using mouse brain S1 cytosolic fraction; microarray: suitable; western blot: 0.1-0.2 μg/mL using whole extract of human epitheloid carcinoma HeLa cell line and also canine kidney MDCK cell line; western blot: 0.2-0.4 μg/mL using whole extract of rat brain S1 cytosolic fraction
• UniProt登錄號: Q6UWE0
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... LRSAM1(90678) ; mouse ... Lrsam1(227738)

说明

一般說明

Tsg101-associated ligase (TAL), contains an N-terminal leucine-rich repeat (LRR), followed by an ezrin-radixin-moesin (ERM) domain, a coiled-coil (CC) region, a sterile alpha motif (SAM) domain and a C-terminal C3HC4-type RING finger domain. Also called leucine-rich repeat and sterile alpha motif-containing protein 1 (LRSAM1), TAL is an E3 ubiquitin ligase. TAL gene is mapped to human chromosome 9.

特異性

Anti-Tal (CS-15) recognizes human, dog, rat, and mouse Tal (84 kDa, may appear as doublet).

免疫原

synthetic peptide encoding amino acids 709-723 located near the C-terminus of human Tal, conjugated to KLH. This sequence is highly conserved in mouse and rat Tal (two amino acids substitution).

應用

Anti-Tal (CS-15) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

生化／生理作用

Tsg101-associated ligase (TAL) plays a key role in cell signaling, cell adhesion and regulates cargo sorting. Reduced expression of Tsg101 shunts active epidermal growth factor receptor (EGFRs) from the normal degradative pathway to a recycling route. Tal, which interacts with Tsg101 associates with and ubiquitylates Tsg101 in a manner that regulates receptor trafficking and budding of viral particles. Tal binds to Tsg101 in a bimodal mode, through an interaction of its CC domain with the steadiness box (SB) of Tsg101, and also via binding of the UEV domain of Tsg101 with two PTAP motifs just upstream of the C-terminal RING domain. Tal interaction with Tsg101 promotes multiple monoubiquitination of Tsg101, rather than polyubiquitination, which leads to the release of Tsg101 from membrane complexes and its inactivation. Mutations in the TAL gene is implicated in Charcot-Marie-Tooth (CMT) syndrome.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

儲存和穩定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.