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一般說明
3,3′,5,5′-四甲基联苯胺(TMB)是辣根过氧化物酶(HRP)偶联物的显色底物。它会产生永久性不可溶解的深蓝色反应产物,可应用于比色定量。
應用
3,3′,5,5′-四甲基联苯胺(TMB)显色液(转印膜用底物)可作为蛋白质免疫印迹分析(western blot)的显色底物。也可用作酶联免疫吸附测定(ELISA)的底物溶液。
3,3′,5,5′-四甲基联苯胺(TMB)细胞膜的液体底物体系:
- 用于酶联免疫斑点法(ELISPOT)分析
- 用作IgG过氧化物酶的底物
- 应用于免疫复合物的可视化
外觀
即用型。
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
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This discussion will highlight some of these methodologies, namely, the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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