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Merck

T0565

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

TMB membrane substrate, chromogenic, liquid

别名:

TMB membrane substrate

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.83

产品名称

用于膜的3,3′,5,5′-四甲基联苯胺(TMB)液体底物系统, ready to use solution

品質等級

形狀

liquid

儲存溫度

2-8°C

一般說明

3,3′,5,5′-四甲基联苯胺(TMB)是辣根过氧化物酶(HRP)偶联物的显色底物。它会产生永久性不可溶解的深蓝色反应产物,可应用于比色定量。

應用

3,3′,5,5′-四甲基联苯胺(TMB)显色液(转印膜用底物)可作为蛋白质免疫印迹分析(western blot)的显色底物。也可用作酶联免疫吸附测定(ELISA)的底物溶液。
3,3′,5,5′-四甲基联苯胺(TMB)细胞膜的液体底物体系:
  • 用于酶联免疫斑点法(ELISPOT)分析
  • 用作IgG过氧化物酶的底物
  • 应用于免疫复合物的可视化

外觀

即用型。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

This discussion will highlight some of these methodologies, namely, the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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