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Merck
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主要文件

SRP6401

Sigma-Aldrich

HGFR/c-MET human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

别名:

AUTS9, MET, RCCP2

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.32

生物来源

human

重组

expressed in HEK 293 cells

标签

8-His tagged

方案

≥95% (SDS-PAGE)

表单

lyophilized

分子量

α-chain calculated mol wt 32.5 kDa
α-chain observed mol wt 45 kDa by SDS-PAGE (reducing) (resulting from glycosylation.)
β-chain 85 kDa
β-chain calculated mol wt 60 kDa

包装

pkg of 20 and 100 μg

杂质

<1 EU/μg endotoxin (LAL test)

UniProt登记号

运输

wet ice

储存温度

−20°C

基因信息

human ... MET(4233)

一般描述

Hepatocyte growth factor receptor (HGFR), also known as mesenchymal-epithelial transition factor (MET), c-Met, and is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGFR protein possesses tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the α and β subunits, which are disulfide linked to form the mature receptor. HGFR is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin. Upon HGF stimulation, HGFR induces several biological responses that collectively give rise to a program known as invasive growth. Abnormal HGFR activation in cancer correlates with poor prognosis, where aberrantly active HGFR triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). HGFR is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express HGFR, However, cancer stem cells are thought to hijack the ability of normal stem cells to express HGFR, and thus become the cause of cancer persistence and spread to other sites in the body. Various mutations in the HGFR gene are associated with papillary renal carcinoma. HGFR mediates a complex program known as invasive growth. Activation of HGFR triggers mitogenesis, and morphogenesis.
The mature form of HGFR is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain.

外形

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

重悬

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

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