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重組細胞
expressed in E. coli
化驗
≥70% (SDS-PAGE)
形狀
buffered aqueous glycerol solution
分子量
~25 kDa
技術
ligand binding assay: suitable
NCBI登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
Escherichia coli IAI39 ... gst> gst
gst(7152360)
一般說明
谷胱甘肽 S-转移酶 (GST) 家族的酶组成了一长串的细胞溶质、线粒体和微粒体蛋白,被用来创建所谓的 GST 基因融合系统。这些蛋白能够与多种底物发生多种反应。在解毒和毒化机制中起角色。GST 标记的大小为 220 个氨基酸,与其他标记(如 myc 或 FLAG-标记)相比相对较大,后者相当小。GST 可用于纯化和检测目的蛋白。在 GST 基因融合系统中,表达介体由编码目的蛋白的基因序列和旁边的 GST 序列组成。诱导表达后,融合蛋白可通过对谷胱甘肽的高亲和力通过亲和层析进行纯化。
應用
无标签 GST 蛋白已在体外肌动蛋白结合试验中使用。它也已被用作TPX2(Xklp2的靶蛋白)的蛋白标签。
外觀
以 50 mM Tris-HCl,pH 7.5,150 mM NaCl,10 mM 谷胱甘肽,0.1 mM EDTA,0.25 mM DTT,0.1 mM PMSF,25% 甘油的形式提供。
準備報告
开封后,分装少量,-70℃ 储存。避免重复处理和多次冻融循环。
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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