SRP3043
FGF-basic human
Animal-component free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
别名:
Catabolin, Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Lymphocyte-activating factor (LAF), Mononuclear Cell Factor (MCF)
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所有图片(1)
About This Item
分類程式碼代碼:
12352202
NACRES:
NA.32
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生物源
human
重組細胞
expressed in E. coli
化驗
≥95% (HPLC)
≥95% (SDS-PAGE)
形狀
lyophilized
效力
≥1x10^7 units/mg
分子量
17.2 kDa
包裝
pkg of 50 μg
技術
cell culture | mammalian: suitable
雜質
<0.1 EU/μg endotoxin, tested
顏色
white to off-white
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... FGF2(2247)
一般說明
FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant human FGF-basic is a 17.2 kDa protein consisting of 154 amino acid residues.
應用
FGF-basic has been used to supplement serum-free DMEM during culture of HeLa cells.
生化／生理作用
FGF-basic (fibroblast growth factor), also referred to as FGF2, activates the ERK (extracellular signal-regulated kinase) cascade and facilitates self-renewal of hESCs (human embryonic stem cells). FGF2 positively regulates cell survival and/or proliferation. It is found to negatively regulate BMP (bone morphogenetic protein) signaling, a pathway that induces non-neuronal differentiation.
序列
AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
外觀
Lyophilized from 5 mM Tris, pH 7.6 + 150mM NaCl.
重構
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8° C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 5% Trehalose) and store in working aliquots at -20° C to -80° C.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
MARCH1 regulates insulin sensitivity by controlling cell surface insulin receptor levels.
Nagarajan A
Nature Communications, 7 (2016)
Boris Greber et al.
The EMBO journal, 30(24), 4874-4884 (2011-11-17)
Human embryonic stem cells (hESCs) can exit the self-renewal programme, through the action of signalling molecules, at any given time and differentiate along the three germ layer lineages. We have systematically investigated the specific roles of three signalling pathways, TGFβ/SMAD2
Fibroblast growth factors.
Ornitz, D.M., et al.
Genome Biology, 2, 30051-300512 (2001)
Shona Kaya et al.
Pharmaceutics, 15(5) (2023-05-27)
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商品
Discover answers to your frequently asked questions about animal component-free, xenobiotic-free, and chemically defined media and reagents for cell culture.
Fibroblast growth factors in cell culture and various growth factors for your research
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