SRP2159

Rev-ErbA, β (RVR) human

recombinant, expressed in insect cells, ≥80% (SDS-PAGE)

• 别名: BD73, EAR-1R, RVR
• 分類程式碼代碼: 12352202
• NACRES: NA.26

属性

• 生物源: human
• 重組細胞: expressed in insect cells
• 化驗: ≥80% (SDS-PAGE)
• 形狀: frozen liquid
• 分子量: ~66.6 kDa
• 包裝: pkg of 5 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 濃度: 350 μg/mL
• 顏色: clear colorless
• NCBI登錄號: NM_005126
• UniProt登錄號: Q14995
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... NR1D2(9975)

说明

生化／生理作用

Nuclear receptors form the largest known family of transcription factors and have a crucial role in nearly all aspects of vertebrate development and adult physiology by transducing the effects of hormones into transcriptional responses. Members of the steroid/thyroid hormone nuclear receptor (NR) superfamily bind specific DNA elements and function as ligand activated transcription factors. This group includes the `orphan receptors′ which have no known ligands in the `classical sense′ and appear to be the ancient progenitors of this receptor superfamily. The Rev-erb family of proteins are orphan members of the receptor superfamily. Two isoforms of the Rev-erb family have been isolated from mammalian genotypes, Rev-erbA[alpha] and Rev-erbA[beta]/RVR. Major differences between the two isoforms occur within the hyper-variable A/B and D regions of the proteins. Both isoforms are expressed in a wide range of tissues and are present in all major organs. Rev-erbA[alpha] mRNA is upregulated during adipocyte differentiation but repressed during myogenesis. These orphan receptors are closely related to the ROR/RZR[alpha] gene family (retinoic acid receptor related orphan receptor) and the Drosophila orphan receptor, E75A, particularly in the DNA-binding domain (DBD) and the putative ligand-binding domain (LBD). RVR and Rev-erbA[alpha] bind as monomers to an asymmetric ( A / T ) 6 RGGTCA motif. The Rev-erb family has also been demonstrated to bind as homodimers to novel HREs consisting of two tandemly arranged AGGTCA motifs, separated by 2 bp with unique 5′ flanking and spacer nucleotides (RevDR-2). Reports on the transcriptional properties of the Rev-erb family were initially conflicting. Rev-erbA[alpha] was first reported to act as a constitutive activator of transcription through its cognate monomeric asymmetric motif. Recently, it has been demonstrated that members of the Rev-erb family are, in fact, dominant repressors of transcription. Rev-erbA[beta] is expressed in the central nervous system, skeletal and dorsal muscles, spleen and mandibular and maxillar processes. During embryogenesis RVR is expressed in the notochord and neural tube, but its function/role during differentiation and mammalian development remains obscure.

外觀

Clear and colorless frozen liquid solution

準備報告

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable