生物源
human
重組細胞
expressed in E. coli
化驗
≥80% (SDS-PAGE)
形狀
frozen liquid
分子量
~33 kDa
包裝
pkg of 10 μg
儲存條件
avoid repeated freeze/thaw cycles
濃度
100 μg/mL
顏色
clear colorless
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... POLR2K(5440)
一般說明
POLR2K (RNA polymerase II subunit K) is located on human chromosome 8q22. Rpb12 is the eukaryotic subunit that is also called as ABC10α. Rpb12 is the general subunit of all three eukaryotic RNA polymerases.
生化／生理作用
RPB12 (also called RPB10 ) is shared between all three RNA polymerases and is homologous to the archaeal P subunit of RNA polymerase. Like RPB10, it is essential for yeast cell viability. RPB12 is also a zinc-binding protein with a canonical tetra-coordinating zinc motif but binds zinc in vitro much less efficiently than the RPB10 subunit. It has been shown to interact with both RPB1 and RPB2 in vitro and the human subunit can functionally substitute for its yeast counterpart.
RPB12/RPC10 (RNA polymerase II subunit K) is required for growth in Saccharomyces cerevisiae. The carboxy-terminal domain (CTD) of RNAP II plays a major role in recruiting many of these activities to the transcriptional machinery.
外觀
Clear and colorless frozen liquid solution
準備報告
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
F Werner et al.
Nucleic acids research, 28(21), 4299-4305 (2000-11-01)
The archaeal and eukaryotic evolutionary domains diverged from each other approximately 2 billion years ago, but many of the core components of their transcriptional and translational machineries still display a readily recognizable degree of similarity in their primary structures. The
Woychik, N.A., et al.
Transcription Mechanisms and Regulation, 227-242 (1994)
Tiling resolution array CGH and high density expression profiling of urothelial carcinomas delineate genomic amplicons and candidate target genes specific for advanced tumors.
Heidenblad M, et al.
BMC Medical Genomics, 1(1), 3-3 (2008)
The archaeal RNA polymerase subunit P and the eukaryotic polymerase subunit Rpb12 are interchangeable in vivo and in vitro.
Reich C, et al.
Molecular Microbiology, 71(4), 989-1002 (2009)
RNA polymerase II conducts a symphony of pre-mRNA processing activities.
Howe K J.
Biochimica et Biophysica Acta, 1577(2), 308-324 (2002)
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