SRP2032

Topo I (C651-765) (C terminal domain) human

recombinant, expressed in insect cells, ≥80% (SDS-PAGE)

• 别名: TOPI
• UNSPSC代码: 12352202
• NACRES: NA.26

属性

• 生物来源: human
• 重组: expressed in insect cells
• 方案: ≥80% (SDS-PAGE)
• 表单: frozen liquid
• 分子量: ~15.2 kDa
• 包装: pkg of 5 μg
• 储存条件: avoid repeated freeze/thaw cycles
• 浓度: 500 μg/mL
• 颜色: clear colorless
• NCBI登记号: NM_003286
• UniProt登记号: P11387
• 运输: dry ice
• 储存温度: −70°C
• 基因信息: human ... TOP1(7150)

说明

生化/生理作用

Human DNA topoisomerase I catalyzes the relaxation of both positive and negative supercoils without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, recombination, chromatin remodeling and other DNA or RNA templating activities. The C terminal domain of DNA topoisomerase I spanning from amino acids 713 to 765 is highly conserved and connected to the core domain by a poorly conserved linker domain (residues 636-713). An active site tyrosine has been characterized at position 723. Mutation of this tyrosine to phenylalanine at position 723 causes topo I to preferentially bind the supercoiled DNA rather than relaxed DNA in the mixture of supercoiled and relaxed DNA.

外形

Clear and colorless frozen liquid solution

制备说明

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

安全信息

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable