SRP2020

TR, β 1 isoform human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• 别名: ERBA-beta, GRTH, NR1A2, THR1, THRB1
• 分類程式碼代碼: 12352202
• NACRES: NA.26

属性

• 生物源: human
• 重組細胞: expressed in E. coli
• 化驗: ≥70% (SDS-PAGE)
• 形狀: frozen liquid
• 分子量: ~53 kDa
• 包裝: pkg of 10 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 濃度: 500 μg/mL
• 顏色: clear colorless
• NCBI登錄號: NM_000461
• UniProt登錄號: P10828
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... THRB(7068)

说明

生化／生理作用

Nuclear receptors form the largest known family of transcription factors and have a crucial role in nearly all aspects of vertebrate development and adult physiology by transducing the effects of hormones into transcriptional responses. The family is defined by two domains: (a) the central, highly conserved, DNA-binding domain (DBD) of approximately 66 amino acids, and (b) the C-terminal, structurally conserved, ligand-binding domain (LBD) of approximately 250 amino acids. The amino-terminal regions are least conserved among nuclear receptor sequences. This domain is highly divergent between TR α and TR β isoforms, which suggests differential roles in transcriptional regulation. In addition, alternative splicing of the TR β gene generates two isoforms, TR β1 and TR β2 with completely different amino-terminal domains. Unliganded TR inhibits the formation of a functional pre-initiation complex, through direct interaction with TBP and transcription factor IIB. In addition, in the absence of ligand TR has been shown to repress transcription through recruitment of a corepressor complex, which also includes Sin3A and histone deacetylase. Ligand binding releases the corepressor complex and recruits a coactivator complex that includes multiple histone acetyltransferases, including a steroid receptor family coactivator, p300/CREB-binding protein–associated factor (PCAF), and CREB binding protein (CBP).

外觀

Clear and colorless frozen liquid solution

準備報告

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable