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生物源
Saccharomyces cerevisiae
重組細胞
expressed in E. coli
化驗
≥80% (SDS-PAGE)
形狀
frozen liquid
分子量
~18.6 kDa
包裝
pkg of 10 μg
儲存條件
avoid repeated freeze/thaw cycles
濃度
300 μg/mL
技術
electrophoretic mobility shift assay: suitable
顏色
colorless to clear
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
Saccharomyces cerevisiae ... GAL4(855828)
生化／生理作用
Transcriptional activity is greatly stimulated by promoter-specific activator proteins. These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain. The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5′-CGGN5(T/A)N5CCG-3′ (5 ). A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. Linking of an acidic synthetic peptide, forming an α-helix (AH), to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure. This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex.
外觀
Clear and colorless frozen liquid solution
準備報告
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
J Ma et al.
Cell, 48(5), 847-853 (1987-03-13)
We describe the activities of a wide array of deletion mutants of GAL4, a yeast transcriptional activator. We identify two short regions of GAL4, each of which activates transcription when fused to the DNA-binding region of the molecule. Very large
M Ptashne et al.
Nature, 346(6282), 329-331 (1990-07-26)
Proteins that activate genes are quite disparate in character; in particular, some work 'universally' and others do not. A simple model can accommodate most of the recently published results.
Commitment and activation at pol II promoters: a tail of protein-protein interactions.
B Lewin
Cell, 61(7), 1161-1164 (1990-06-29)
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