SRP0717

NEK8, Active

PRECISIO^® Kinase, recombinant

• UNSPSC代码: 12352202
• NACRES: NA.77

属性

• 重组: expressed in baculovirus infected Sf9 cells
• 方案: 70-95% (SDS-PAGE)
• 表单: frozen liquid
• 比活: 5.0-7.0 nmol/min·mg
• 分子量: ~66 kDa
• 浓度: 0.05 μg/μL
• 登记号: NM_178170
• 运输: dry ice
• 储存温度: −70°C
• 基因信息: human ... NEK8(284086)

说明

应用

Useful for kinase assay and Western blot.

生化/生理作用

Recombinant human NEK8 (1-322) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK8 or NIMA-related kinase 8 is a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans which may play a role in cell cycle progression from G2 to M phase. NEK8 is part of a complex of ciliary proteins required for renal and cardiovascular development ⁽¹⁾. NEK8 interacts with polycystin signal transduction pathways and may control the targeting of these ciliary proteins ⁽²⁾.

重悬

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

储存及稳定性

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

法律信息

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable