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容量
0.2 mg loading(0.2 mg D-Ribulose 1,5-Diphosphate Carboxylase (Rubisco))
储存温度
2-8°C
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一般描述
RuBisCO(核酮糖-1,5-二磷酸羧化酶/加氧酶)是植物中最丰富的蛋白质,而且可能也是地球上最丰富的蛋白质。RuBisCO是一种酶,在Calvin循环中催化碳固定反应的第一个关键步骤,通过碳固定反应可将大气中游离的二氧化碳转化为生物体内储能分子,如蔗糖分子。RuBisCO是绿叶进行光合碳同化的关键酶,同时也是植物蛋白组学研究的主要障碍。RuBisCO约占绿叶中蛋白总量的40%,可干扰蛋白质组学研究,如LC-MS/MS和二维凝胶电泳。为了从其他植物蛋白中特异和有效分离RuBisCO,Sigma开发了基于IgY抗体与微球交联的新型免疫亲和离心柱,可特异性去除植物提取物中的RuBisCO蛋白。
应用
Seppro® Rubisco,离心柱(容量:∼0.2 mg Rubisco)可用于植物蛋白质组学研究,通过液相色谱来有效进行针对植物组织提取物中RuBisCO(核酮糖-1,5-二磷酸羧化酶/加氧酶)的IgY介导的免疫亲和去除。
法律信息
Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
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