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生物源
alpaca
重組細胞
expressed in E. coli
共軛
unconjugated (6*his tag and one cys were added at the C terminal of the VHH)
抗體表格
affinity purified immunoglobulin
抗體產品種類
secondary antibodies
無性繁殖
polyclonal
形狀
lyophilized solid
分子量
14 kDa
物種活性
Alpaca, mouse
技術
ELISA: 1:10000-1:50000
western blot: 1:10000-1:50000
尺寸
0.1 mL
顏色
colorless
同型
VHH
運輸包裝
wet ice
儲存溫度
2-8°C
一般說明
Alpaca Anti-Mouse IgG(H+L) single domain Antibody is designed to specifically detect mouse IgG(H+L). It is a single domain recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse IgG(H+L) single domain Antibody reacts with the heavy chain and light chain of mouse IgG selectively but not with rabbit, human, cynomolgus, rat, goat IgG.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
特異性
Mouse IgG(H+L)
Isotype: VHH domain of alpaca IgG2b/2c
Isotype: VHH domain of alpaca IgG2b/2c
免疫原
Recombinant Alpaca single variable domain secondary nano antibody
包裝
100 μg in Glass bottle
外觀
Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose
重構
Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL
儲存和穩定性
After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C
其他說明
No cross-reactivity with mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
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訊號詞
Warning
危險聲明
危險分類
Aquatic Chronic 3 - Skin Sens. 1
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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