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Merck

SAB4200726

Sigma-Aldrich

Anti-Myosin (Smooth) antibody, Mouse monoclonal

clone hSM-V, purified from hybridoma cell culture

别名:

Anti-SM

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About This Item

分類程式碼代碼:
41116158

生物源

mouse

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

hSM-V, monoclonal

物種活性

quail, rabbit, rat, human, sheep, bovine, mouse, porcine, canine

濃度

~1.0 mg/mL

技術

immunoblotting: suitable
immunofluorescence: suitable
immunohistochemistry: 10-20 μg/mL using methacarn -fixed paraffin-embedded human tonsil sections
immunoprecipitation (IP): suitable

同型

IgG1

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... MYH11(4629)

一般說明

Anti-Myosin (Smooth) antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hSM-V hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with human uterus smooth muscle extract. Myosin together with actin belong to a family of contractile proteins. Myosin contains two heavy chains (~200 kDa each) and four light chains (15-26 kDa). The Myosin molecules which consist of two major regions: tail (rod) and head, aggregate through the tail region into filaments and interact through the head region with actin and with ATP.

免疫原

human uterus smooth muscle extract

應用

Anti-Myosin (Smooth) antibody, Mouse monoclonal has been used in various immunochemical assays, including immunohistochemistry, immunoblotting, immunofluorescence, immunoprecipitation and fluorescence-activated cell sorting (FACS) / flow cytometry.

生化/生理作用

Myosin is involved in a variety of cellular processes including secretion, cytoplasmic streaming, locomotion, phagocytosis and cytokinesis. This antibody can be a useful tool in the identification and localization of smooth muscle elements, in the diagnosis and classification of mesenchymal tumors and in myosin expression studies in normal and malignant cell proliferations.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Complete Characterization of Cardiac Myosin Heavy Chain (223 kDa) Enabled by Size-Exclusion Chromatography and Middle-down Mass Spectrometry
Jin Y, et al.
Analytical Chemistry, 89, 4922-4922 (2017)

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