SAB4200480

Anti-Kindlin-1 antibody, Mouse monoclonal

clone 4A5.14, purified from hybridoma cell culture

• 别名: Monoclonal Anti-C20orf42, Monoclonal Anti-DTGCU2, Monoclonal Anti-FERMT1 URP1, Monoclonal Anti-KIND1, Monoclonal Anti-Kindlin-1 antibody produced in mouse, Monoclonal Anti-UNC112A, Monoclonal Anti-fermitin family member 1
• UNSPSC代码: 12352203
• NACRES: NA.41

属性

• 生物来源: mouse
• 偶联物: unconjugated
• 抗体形式: purified from hybridoma cell culture
• 抗体产品类型: primary antibodies
• 克隆: 4A5.14, monoclonal
• 表单: buffered aqueous solution
• 分子量: antigen ~77 kDa
• 种属反应性: human
• 浓度: ~1.0 mg/mL
• 技术: flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: 2.5-5.0 μg using SW-48 cells; immunoprecipitation (IP): suitable; western blot: 2.0-4.0 μg/mL using SW-48 total cell extracts
• 同位素/亚型: IgG2a
• UniProt登记号: Q9BQL6
• 运输: dry ice
• 储存温度: −20°C
• 靶向翻译后修饰: unmodified
• 基因信息: human ... FERMT1(55612)

说明

一般描述

Monoclonal Anti-Kindlin-1 (mouse IgG2a isotype) is derived from the hybridoma 4A5.14 produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Kindlin-1 is a focal adhesion protein, which belongs to the family of Kindlin proteins. It is highly expressed in epithelial cells, including keratinocytes. Kindlin-1 is localized to the focal adhesions. A splice variant potentially producing a truncated protein has been detected in the kidney, colon and small intestine. Kindlin-1 comprises a bipartite 4.1 protein, ezrin, radixin, moesin (FERM) domain with four subdomains (F0, F1, F2, and F3), which is found in many proteins. The KIND1 gene is present at human chromosome location 20p12.3.

特异性

Monoclonal Anti-Kindlin-1 recognizes human Kindlin-1.

免疫原

human Kindlin-1 recombinant fusion protein.

应用

Monoclonal Anti-Kindlin-1 antibody produced in mouse may be used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• immunohistochemistry
• flow cytometry

生化/生理作用

Kindlin-1 is implicated in the activation of integrin adhesion receptors and the reorganization of the actin cytoskeleton. Deficiency of kindlin-1 is linked to Kindler syndrome, an autosomal recessive genodermatosis characterized by skin blistering, progressive skin atrophy, photosensitivity and carcinogenesis. This gene is also implicated in breast cancer, lung metastasis and lung tumorigenesis. The 4.1 protein, ezrin, radixin, moesin (FERM) domain of Kindlin-1 is involved in cytoskeletal organization. In colon cancer, Kindlin-1 is identified as a potential marker. Kindlin-1 modulates epidermal growth factor receptor signaling.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

储存及稳定性

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 2
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable