SAB4200379

Anti-JMY antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 别名: Anti-Junction mediating and regulatory protein, Anti-p53 cofactor
• UNSPSC代码: 12352203
• NACRES: NA.41

属性

• 生物来源: rabbit
• 偶联物: unconjugated
• 抗体形式: affinity isolated antibody
• 抗体产品类型: primary antibodies
• 克隆: polyclonal
• 表单: buffered aqueous solution
• 分子量: antigen ~120 kDa
• 种属反应性: human
• 浓度: ~1.0 mg/mL
• 技术: immunoprecipitation (IP): 5-10 μg using lysates of human WiDr cells.; indirect immunofluorescence: 1-2 μg/mL using human HeLa cells.; western blot: 0.5-1.0 μg/mL using whole extracts of human WiDr cells
• UniProt登记号: Q8N9B5
• 运输: dry ice
• 储存温度: −20°C
• 靶向翻译后修饰: unmodified
• 基因信息: human ... JMY(133746)

说明

一般描述

Junction mediating and regulatory protein (JMY) is identified as a transcriptional co-activator of p53 and is a multifunctional actin nucleation factor. JMY contains Wiskott-Aldrich syndrome protein (WASp)-homology 2 (WH2) domains in its C-terminus. The JMY gene is mapped to human chromosome location 5q14.1.

特异性

Anti-JMY recognizes human JMY.

免疫原

synthetic peptide corresponding to the C-terminal region of human JMY, conjugated to KLH.

应用

Anti-JMY antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence.

生化/生理作用

Junction mediating and regulatory protein (JMY) acts as a nucleation promoting factor by activating actin-related proteins-2/3 (ARP2/3), creating branched filament networks, and also by assembling unbranched filaments directly through a Spire-like mechanism. Overexpression of JMY increases motility while its loss slows cell migration. DNA damage causes JMY to accumulate in the nucleus, where it enhances p53-dependent transcription of pro-apoptotic genes. The activities of JMY as a p53 co-factor or as an actin nucleation factor are regulated by its subcellular localization.

外形

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

储存及稳定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 10 - Combustible liquids
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable