生物源
rabbit
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen ~43 kDa
物種活性
human
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
技術
western blot: 1-2 μg/mL using extracts of Raji cells
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... LPXN(9404)
一般說明
Leupaxin gene is mapped to human chromosome 11q12.1. Leupaxin belongs to the paxillin protein family It is mainly expressed in macrophages, lymphoid tissues and hematopoietic cells. Leupaxin comprises two types of protein interaction domains, LIM domain and leucine−aspartate (LD) motifs. LIM domains mediate localization to focal contacts. LD motifs facilitates focal adhesion kinase (FAK) and vinculin binding, resulting in the localization of focal adhesion kinase (FAK) to focal adhesions. The C-terminal LIM domains in leupaxin share 70% amino acid identity with paxillin. The N-terminal region of leupaxin comprises three short stretches of 13 amino acids that share 70− 90% similarity with paxillin LD motifs.
特異性
Anti-Leupaxin (N-terminal) specifically recognizes human leupaxin.
應用
Anti-Leupaxin antibody produced in rabbit has been used in immunoblotting and proximity ligation assay.
生化/生理作用
Leupaxin in association with a second focal adhesion kinase (FAK) family member, PYK2, acts a cell type-specific signaling complex regulator in leukocytes. It acts as a tyrosine kinase substrate in lymphoid cells and thus, may function in and be regulated by tyrosine kinase activity. Leupaxin facilitates adhesion and invasion of prostate carcinoma cells. It can form a complex with PYK2 and PTP-PEST in various cells such as monocytes and osteoclasts and in prostate cancer cells. In osteoclasts, leupaxin acts as a key nucleating component of the osteoclast podosomal signaling complex in the adhesion zone.
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
儲存和穩定性
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
閃點(°F)
Not applicable
閃點(°C)
Not applicable
Leupaxin stimulates adhesion and migration of prostate cancer cells through modulation of the phosphorylation status of the actin-binding protein caldesmon
Oncotarget, 6(15), 13591-13591 (2015)
Structural basis for the interaction between Pyk2-FAT domain and leupaxin LD repeats
Biochemistry, 55(9), 1332-1345 (2016)
Leupaxin promotes bladder cancer proliferation, metastasis, and angiogenesis through the PI3K/AKT pathway
Cellular Physiology and Biochemistry, 47(6), 2250-2260 (2018)
Leupaxin, a novel coactivator of the androgen receptor, is expressed in prostate cancer and plays a role in adhesion and invasion of prostate carcinoma cells
Molecular Endocrinology, 22(7), 1606-1621 (2008)
Oncotarget, 6(15), 13591-13606 (2015-06-17)
The focal adhesion protein leupaxin (LPXN) is overexpressed in a subset of prostate cancers (PCa) and is involved in the progression of PCa. In the present study, we analyzed the LPXN-mediated adhesive and cytoskeletal changes during PCa progression. We identified
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