生物源
rabbit
共軛
peroxidase conjugate
抗體表格
affinity isolated antibody
抗體產品種類
secondary antibodies
無性繁殖
polyclonal
形狀
lyophilized powder
物種活性
chicken
技術
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
相关类别
一般說明
Chicken IgG (immunoglobulin G) is also referred to as IgY. IgY is an antibody produced in birds. IgY is similar to mammalian IgG but differs in some characteristics from IgG. IgY does not bind with mammalian complements nor rheumatoid factor, which are present in mammalian serum and anti-mammalian IgG antibodies. During oogenesis in chicken, IgY can pass across the follicular epithelium of the ovary and aggregate in the yolk. The heavy chain of IgY and mammalian IgG has a molecular weight of 68kDa and 50kDa, respectively. The structure of mammalian IgG is considered more stable than that of IgY. IgG antibodies, which often cross-react to IgG from different mammalian species, exhibit low cross-reactivity to IgY. Thus, IgY is beneficial when it is used as a biochemical/clinical reagent.
特異性
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab′)2 and Chicken Serum. No reaction was observed against Chicken IgG F(c).
免疫原
Chicken IgG F(ab′)2 fragment
物理性質
Antibody format: IgG
外觀
Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
重構
Reconstitute with 1.0 mL deionized water (or equivalent).
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Production and purification of Fab? fragments from chicken egg yolk immunoglobulin Y (IgY)
Journal of Immunological Methods, 162(2), 155-164 (1993)
Molecular stability of chicken and rabbit immunoglobulin G.
Bioscience, Biotechnology, and Biochemistry, 56(2), 270-274 (1992)
Immunoglobulins of the non-galliform birds: antibody expression and repertoire in the duck.
Developmental and Comparative Immunology, 30(1-2), 93-100 (2006)
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