SAB1409315

Monoclonal Anti-MAP3K4 antibody produced in mouse

clone 6A12, purified immunoglobulin, buffered aqueous solution

• 别名: FLJ42439, KIAA0213, MAPKKK4, MEKK4, MTK1, PRO0412
• UNSPSC代码: 12352203
• NACRES: NA.41

属性

• 生物来源: mouse
• 偶联物: unconjugated
• 抗体形式: purified immunoglobulin
• 抗体产品类型: primary antibodies
• 克隆: 6A12, monoclonal
• 表单: buffered aqueous solution
• 分子量: antigen 36.63 kDa
• 种属反应性: human
• 技术: indirect ELISA: suitable; proximity ligation assay: suitable; western blot: 1-5 μg/mL
• 同位素/亚型: IgG1κ
• NCBI登记号: NM_005922
• UniProt登记号: Q9Y6R4
• 运输: dry ice
• 储存温度: −20°C
• 靶向翻译后修饰: unmodified
• 基因信息: human ... MAP3K4(4216)

说明

一般描述

The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described. (provided by RefSeq)

免疫原

MAP3K4 (NP_005913, 1201 a.a. ~ 1300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
AASRPSPSGGDSVLPKSISSAHDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPRGDSSGSTRRSWELRTLISQSKDTASKLGPIEAIQKS

外形

Solution in phosphate buffered saline, pH 7.4

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 3
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable