S7570

SGK2 Active human

≥75% (SDS-PAGE), aqueous glycerol solution, recombinant, expressed in E. coli

• 别名: Serum/Glucocorticoid-Regulated Kinase 2
• MDL號碼: MFCD07371188
• 分類程式碼代碼: 12352200
• NACRES: NA.32

属性

• 重組細胞: expressed in E. coli
• 品質等級: 200
• 化驗: ≥75% (SDS-PAGE)
• 形狀: aqueous glycerol solution
• 比活性: 100 units/mg protein (Bradford)
• 分子量: protein apparent mol wt ~66 kDa
• UniProt登錄號: Q9HBY8
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... SGK2(10110)

说明

應用

Kinase activity is measured as the molar amount of phosphate incorporated into the Akt/SGK substrate peptide (RPRAATF), per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

生化／生理作用

SGK2 is a member of the serum- and glucocorticoid-induced kinases (SGK) which are serine-threonine kinases and belong to the "AGC" kinase subfamily, which includes protein kinases A, G, and C and has SGK2 enhances Na⁺/K⁺-ATPase activity in a variety of cells. In addition, SGK2 mimics the function of SGK1 and SGK3 and participates in the regulation of renal epithelial Na⁺ channel ENaC activity.

單位定義

One unit will incorporate one nanomole of phosphate into the AKT/SGK substrate peptide(RPRAATF) per minute at 30 °C and pH 7.2 using a final concentration of 50 μM [32P] ATP.

外觀

Supplied at 50 μL in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable