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Merck

RAB0143

Sigma-Aldrich

人类 Dkk-1 ELISA 试剂盒

for serum, plasma, cell culture supernatant and urine

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About This Item

分類程式碼代碼:
41116158
NACRES:
NA.84

物種活性

human

包裝

kit of 96 wells (12 strips x 8 wells)

技術

ELISA: suitable
capture ELISA: suitable

輸入

sample type serum
sample type urine
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 100 pg/mL
standard curve range: 122.9-30000 pg/mL

檢測方法

colorimetric

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

human ... DKK1(22943)

一般說明

The human Dkk-1 (hDkk-1) gene, a transcriptional target of the p53 tumor suppressor, encodes a powerful inhibitor of the Wnt signaling pathway and regulates the spatial patterning/morphogenesis of the mammalian central nervous system.The Dkk-1 gene, also called Dickkopf-1 , is a prototype member of a recently identified gene family that encodes secreted glycoproteins that control cell fate and neural patterning during embryonic development. This gene is located on human chromosome 10q.

免疫原

Recombinant Human DKK1

應用

请参考Protocol了解详情。

生化/生理作用

Evidence strongly indicates that Dkk-1 protein functions as a potent inhibitor of the Wnt signaling pathway to enable appropriate positioning and development of the embryonic brain and other organ structures. hDkk-1 protein also has a pro-apoptotic function.

其他說明

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)化学品安全说明书

  • RABELADDELISA 5X Assay/Sample Diluent Buffer D (Item K)化学品安全说明书

  • RABSTOP3ELISA Stop Solution (Item I)化学品安全说明书

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)化学品安全说明书

  • RABWASH420X Wash Buffer (Item B)化学品安全说明书

象形圖

Corrosion

訊號詞

Warning

危險聲明

防範說明

危險分類

Met. Corr. 1

儲存類別代碼

8A - Combustible corrosive hazardous materials


分析证书(COA)

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Human Dkk-1, a gene encoding a Wnt antagonist, responds to DNA damage and its overexpression sensitizes brain tumor cells to apoptosis following alkylation damage of DNA
Shou J, et al.
Oncogene, 21(6), 878-878 (2002)
Tumors of the Central Nervous System (2011)
Mariusz L Hartman et al.
PloS one, 9(4), e95157-e95157 (2014-04-16)
The diversity of functional phenotypes observed within a tumor does not exclusively result from intratumoral genetic heterogeneity but also from the response of cancer cells to the microenvironment. We have previously demonstrated that the morphological and functional phenotypes of melanoma

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