R8029

Anti-RAD17 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 别名: Anti-S.cerevisiae homolog of RAD24
• MDL编号: MFCD08705510
• UNSPSC代码: 12352203
• NACRES: NA.41

属性

• 生物来源: rabbit
• 偶联物: unconjugated
• 抗体形式: affinity isolated antibody
• 抗体产品类型: primary antibodies
• 克隆: polyclonal
• 表单: buffered aqueous solution
• 分子量: antigen 70 kDa (also recognizes 75 kDa)
• 种属反应性: human
• 浓度: ~1 mg/mL
• 技术: immunoprecipitation (IP): suitable; indirect immunofluorescence: 5-10 μg/mL using HEK293-T cells fixed with methanol-acetone; microarray: suitable; western blot: 0.5-1.0 μg/mL using HeLa cell lysates
• UniProt登记号: O75943
• 运输: dry ice
• 储存温度: −20°C
• 靶向翻译后修饰: unmodified
• 基因信息: human ... RAD17(5884)

说明

一般描述

RFC-like DNA damage sensor (RAD17)/checkpoint clamp loader component, a homolog of the Schizosaccharomyces pombe checkpoint gene, rad17 is located on human chromosome 5q13.2. This gene is abundantly expressed in the testis.

特异性

Anti RAD17 (C-terminal) specifically recognizes RAD17 (70 kDa and 75 kDa).

免疫原

synthetic peptide corresponding to amino acids 623-640 of human Rad17 (isoform 2), conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is present in isoforms 1-4 of RAD17.

应用

Anti-RAD17 (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

生化/生理作用

RFC-like DNA damage sensor (RAD17)/checkpoint clamp loader component participates in DNA replication, repair and modification or chromatin remodeling. Phosphorylation of RAD17 is a critical event during checkpoint signaling in DNA-damaged cells. RAD17 has been reported in colon, breast and non-small cell lung carcinoma. It may also participate in cell cycle control.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

储存及稳定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 3
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable
• 个人防护装备: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)