R6878

Monoclonal Anti-phospho-Retinoblastoma (pSer⁷⁹⁵) antibody produced in mouse

~2 mg/mL, clone RB-10, purified immunoglobulin, buffered aqueous solution

• 别名: Anti-phospho-Rb (pSer⁷⁹⁵)
• MDL號碼: MFCD03457570
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: RB-10, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen 110 kDa
• 物種活性: mouse, rat, human, bovine, hamster
• 濃度: ~2 mg/mL
• 技術: immunohistochemistry: suitable using 3% paraformaldehyde fixation, 0.15% Triton X-100 permeabilization; indirect ELISA: suitable; microarray: suitable; western blot: 2-4 μg/mL using a whole cell extract of human melanoma cell line G-361
• 同型: IgG1
• UniProt登錄號: P06400
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: phosphorylation (pSer795)
• 基因資訊: human ... RB1(5925) ; mouse ... Rb1(19645) ; rat ... Rb1(24708)

说明

一般說明

Monoclonal Anti-phospho-Retinoblastoma (Rb) (pS795) (mouse IgG1 isotype) is derived from the RB-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic phospho peptide corresponding to the C-terminal amino acids of human retinoblastoma protein, conjugated to KLH. Retinoblastoma proteins p107, p110 (also designated Rb, or pRb) and p130, referred to collectively as pocket proteins, constitute a nuclear protein family that share a common structural unit (the pocket) dedicated to binding certain transcription factors and thereby regulating cellular proliferation. Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with conserved LXCXE motif.

免疫原

synthetic phosphopeptide corresponding to the C-terminal amino acids 791-804 (pSer⁷⁹⁵) of human retinoblastoma protein conjugated to KLH.

應用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Monoclonal Anti-phospho-Retinoblastoma (pSer⁷⁹⁵) antibody produced in mouse has been used in:

• immunoblotting
• immunocytochemistry
• immunofluorescence
• fluorescence-activated cell sorting (FACS) assays
• enzyme linked immunosorbent assay (ELISA)

生化／生理作用

Retinoblastoma (Rb) exerts its function by repressing the transcription of cellular genes required for DNA replication and cell division. It can be phosphorylated at multiplicity of sites in vitro by cell division cycle protein 2 (Cdc2), cyclin-dependent kinase (Cdk2) and Cdk4/Cdk6 kinase complexes. Phosphorylation of pS⁷⁹⁵ is required to inactivate Rb imposed growth suppression.

外觀

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids