P9498

Monoclonal Anti-PIAS-x antibody produced in mouse

clone PIASX-116, purified immunoglobulin, buffered aqueous solution

• 别名: Anti-Protein Inhibitor of Activator STAT
• MDL编号: MFCD08705595
• UNSPSC代码: 12352203
• NACRES: NA.41

属性

• 生物来源: mouse
• 偶联物: unconjugated
• 抗体形式: purified immunoglobulin
• 抗体产品类型: primary antibodies
• 克隆: PIASX-116, monoclonal
• 表单: buffered aqueous solution
• 分子量: antigen ~75 kDa
• 种属反应性: human
• 技术: immunocytochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using proteasome fraction II of HeLa cells
• 同位素/亚型: IgG1
• UniProt登记号: O75928
• 运输: dry ice
• 储存温度: −20°C
• 靶向翻译后修饰: unmodified
• 基因信息: human ... PIAS2(9063)

说明

一般描述

Monoclonal Anti-PIAS-x (mouse IgG1isotype) is derived from the PIASX-116 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. Protein inhibitor of activated STAT 2 (PIAS2/PIAS-x), a small ubiquitin-like modifier (SUMO) E3 ligase contains a zinc binding motif and a highly acidic region. This gene is located on human chromosome 18q21.1.

特异性

Monoclonal Anti-PIAS-x recognizes human PIAS-x.

免疫原

peptide corresponding to amino acids 26-40 of human PIAS-x.

应用

Monoclonal Anti-PIAS-x antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunocytochemistry
• immunoblotting

生化/生理作用

Protein inhibitor of activated STAT2 (PIAS2) plays a key role in SUMO modification (SUMOylation) of several host and viral proteins. PIAS-x acts as a transcriptional co-repressor of STAT4. It may participate in controlling the chromatin structure. Upregulation or suppression of this gene can control the stability of the hepatitis C virus (HCV) core, NS3 and NS5A proteins. Hence PIAS-x is considered as the restriction factor for HCV replication.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

储存及稳定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recom-mended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.