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Merck

P3803

Sigma-Aldrich

苯酚:氯仿:异戊醇25:24:1,10mM Tris饱和溶液,pH8.0,1mM EDTA

for molecular biology

别名:

DNA extraction

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About This Item

分類程式碼代碼:
12190000
NACRES:
NA.52

等級

for molecular biology

品質等級

化驗

≥99.0%

形狀

liquid

pH值

6.5-6.9(pH of phenolic phase)

應用

agriculture

儲存溫度

2-8°C

應用

使用苯酚:氯仿:异戊醇溶液提取,可以从核酸中去除蛋白质。对于核酸粗提混合物,在使用苯酚:氯仿:异戊醇溶液提取前,最好先用一系列蛋白水解酶消解。尽管蛋白质能够被苯酚有效变性,核酸酶活性并未被完全抑制。因此需加入少量异戊醇,进一步确保抑制核酸酶活性。请查阅我们的产品信息表,获得从核酸中去除蛋白质的实验方案。

警告

如果苯酚接触到皮肤,会很快烧伤。因此,使用者需要穿着合适的实验服,包括手套和眼睛护具。如果直接接触到皮肤,立刻用大量水冲洗,并且寻求医学救助。

其他說明

该产品的保质期是两年。

推薦產品

进行DNA提取时,可以用Tris缓冲液平衡,将苯酚相的pH调整到8.0。Sigma提供专门用于DNA提取的P2069产品。该产品包括了平衡缓冲液,能够方便地调节pH值。

相關產品

产品编号
说明
价格

訊號詞

Danger

危險分類

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

標靶器官

Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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