P0498

Anti-PRL-3 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 别名: Anti-PTP4A3, Anti-Phosphatase of Regenerating Liver
• MDL號碼: MFCD07370459
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: IgG fraction of antiserum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~25 kDa
• 物種活性: human
• 技術: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:250-1:500 using protease digested tissue sections from human heart by biotin/ExtrAvidin^®-peroxidase staining; immunoprecipitation (IP): 0.5-1 μL using preparation containing recombinant human PRL-3; western blot (chemiluminescent): 1:500-1:1,000 using whole extract of human HepG2 cells
• UniProt登錄號: O75365
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PTP4A3(11156) ; mouse ... Ptp4a3(19245)

说明

一般說明

Phosphatase of regenerating liver (PRL-3), also known as protein tyrosine phosphatase type IVA, member 3 (PTP4A3) is a dual-specificity phosphatase. It belongs to the phosphatases of regenerating liver family. The PTP4A3 gene is localized on human chromosome 8q24.3.

特異性

Anti-PRL-3 recognizes human phosphatase of regenerating live (PRL)-3 and does not cross-react with human PRL-1 and PRL-2.

免疫原

Synthetic peptide corresponding to amino acid residues of human PRL-3 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse.

應用

Anti-PRL-3 antibody produced in rabbit has been used in western blotting and immunohistochemistry.

生化／生理作用

Phosphatase of regenerating liver (PRL-3) promotes epithelial-mesenchymal transition. It also plays an essential role in cancer cell migration, invasion and metastasis. It dephosphorylates serine, threonine and tyrosine residues. The protein is overexpressed in various cancers. PTP4A3 associates with transcription factors and regulates gene transcription. In addition, it also regulates breast cancer cell proliferation and invasion. Thus, this protein acts as a potent therapeutic target for breast cancer.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

儲存和穩定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

法律資訊

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)