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Merck

N6514

Sigma-Aldrich

神经氨酸酶 来源于霍乱弧菌

Type II, buffered aqueous solution, 8-24 units/mg protein (Lowry, using NAN-lactose)

别名:

受体破坏酶, 唾液酸酶, 神经氨酸苷酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

Vibrio cholerae

品質等級

種類

Type II

形狀

buffered aqueous solution

比活性

8-24 units/mg protein (Lowry, using NAN-lactose)

異物活動

Protease and NAN-aldolase, present

儲存溫度

2-8°C

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相关类别

一般說明

神经氨酸酶是一种促进流感病毒从感染细胞释放并促进病毒传播的水解酶。
量热法研究表明,神经氨酸酶中心催化结构域侧翼的两个凝集素样结构域可作为含唾液酸底物的识别和结合位点。

應用

来自霍乱弧菌的神经氨酸酶已被用于一项描述五步纯化方法的研究中。 它还被用于一项探究白血病L1210肿瘤细胞修饰的研究中。
神经氨酸酶被用作糖缀合物分布和底物特异性研究的细胞表面探针。

品質

不含防腐剂。

單位定義

除特殊规定,在37 °C pH 5.0条件下,一个单位可在每分钟内从NAN-乳糖或牛颌下粘蛋白中释放将1.0 μ摩尔的N-乙酰神经氨酸。价格是基于使用NAN-乳糖作为底物的单位。

外觀

含有0.15 M 氯化钠及及4 mM 叠氮化钠并经0.2 μm滤器过滤的pH 5.5 50 M 醋酸钠中的溶液。

準備報告

通过亲和色谱度我们的III类进行进一步纯化。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Increase of leukemia L1210 immunogenicity by Vibrio cholerae neuraminidase treatment.
J G Bekesi et al.
Cancer research, 31(12), 2130-2132 (1971-12-01)
Paul Clark et al.
Viruses, 12(10) (2020-10-24)
Polyomaviruses are small, non-enveloped DNA tumor viruses that cause serious disease in immunosuppressed people, including progressive multifocal leukoencephalopathy (PML) in patients infected with JC polyomavirus, but the molecular events mediating polyomavirus entry are poorly understood. Through genetic knockdown approaches, we
Hirokazu Kai et al.
Bioorganic & medicinal chemistry, 20(8), 2739-2746 (2012-03-14)
A selective and potent inhibitor of neuraminidases, a hydrolase that is responsible for processing sialylated glycoconjugates, is a promising drug candidate for various infective diseases. The current study demonstrates that the use of an aglycone-focused library of 2-difluoromethylphenyl α-sialosides is
Ibrahim Moustafa et al.
The Journal of biological chemistry, 279(39), 40819-40826 (2004-07-01)
Vibrio cholerae neuraminidase (VCNA) plays a significant role in the pathogenesis of cholera by removing sialic acid from higher order gangliosides to unmask GM1, the receptor for cholera toxin. We previously showed that the structure of VCNA is composed of
Julia Moh-Klaren et al.
Transfusion, 57(11), 2571-2577 (2017-06-24)
Red blood cell (RBC) Thomsen-Friedenreich antigen exposure (T activation) in infants with necrotizing enterocolitis (NEC) has occasionally been associated with posttransfusional intravascular hemolysis thought to be due to anti-T antibodies in the donor plasma. We describe an infant with NEC

商品

Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.

实验方案

Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.

在测定神经氨酸酶活性时,使用了一种以N-乙酰新胺酸为酶底物的比色法进行测定。神经氨酸酶是一种可促进流感病毒从感染细胞中释放的水解酶。

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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