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Merck

N5759

Sigma-Aldrich

4-硝基苯基 β- D -纤维二糖

chromogenic, ≥98% (TLC), powder

别名:

p -硝基苯基 β- D -纤维二糖

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About This Item

经验公式(希尔记法):
C18H25NO13
CAS号:
分子量:
463.39
Beilstein:
100234
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.32

product name

4-硝基苯基 β- D -纤维二糖, ≥98% (TLC)

品質等級

化驗

≥98% (TLC)

形狀

powder

溶解度

water: 49.00-51.00 mg/mL

儲存溫度

2-8°C

SMILES 字串

OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@@H](O[C@@H]2CO)Oc3ccc(cc3)[N+]([O-])=O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C18H25NO13/c20-5-9-11(22)12(23)14(25)18(30-9)32-16-10(6-21)31-17(15(26)13(16)24)29-8-3-1-7(2-4-8)19(27)28/h1-4,9-18,20-26H,5-6H2/t9-,10-,11-,12+,13-,14-,15-,16-,17-,18+/m1/s1

InChI 密鑰

IAYJZWFYUSNIPN-KFRZSCGFSA-N

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相关类别

應用

4-硝基苯基β-D-吡喃木糖苷已用作底物,以测定β-D-纤维二糖苷酶或纤维二糖水解酶(胞外纤维素酶)在微生物群落中的活性。它还可用作底物来研究内切葡聚糖酶J30的活性。

生化/生理作用

4-硝基苯基 β-D-吡喃木糖苷是一种纤维三糖类似物,和常用于检测纤维素酶活性的显色底物。外切葡聚糖酶、内切葡聚糖酶和β -葡糖苷酶可水解4-硝基苯基 β-D-吡喃木糖苷,以形成p-硝基酚(PNP)。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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R M Gardner et al.
Journal of bacteriology, 169(10), 4581-4588 (1987-10-01)
An exo-beta-1,4-glucanase (Exo A) from Ruminococcus flavefaciens FD-1 was purified to homogeneity and characterized. Enzyme activity was monitored during purification by using the substrate p-nitrophenyl-beta-D-cellobioside (NPC). Over 85% of the NPC activity was found to be extracellular once the filter
S J Han et al.
The Journal of biological chemistry, 270(43), 26012-26019 (1995-10-27)
Bacillus sp. D04 secreted a bifunctional cellulase that had a molecular weight of 35,000. This cellulase degraded Cm-cellulose, cellotetraose, cellopentaose, p-nitrophenyl-beta-D-cellobioside, and avicel PH101. Based on the high performance liquid chromatography analysis of the degradation products, this cellulase randomly cleaved
M Fukumura et al.
Bioscience, biotechnology, and biochemistry, 59(1), 47-50 (1995-01-01)
The thermal denaturation process of Clostridium stercorarium F-9 xylanase (XynB) was studied by monitoring remaining activity and recovered activity of the enzyme. At pH 5.5, aggregation occurred rapidly after the thermal denaturation initiated. The aggregated protein could be dissolved in
Effects of microplastic fibers on soil aggregation and enzyme activities are organic matter dependent
Liang Y, et al.
Frontiers in environmental science., 97 null
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European journal of biochemistry, 253(1), 101-106 (1998-05-13)
The interaction of cellobiose dehydrogenase (CDH) with cellobiohydrolase I (CBH I) in cellulose-grown cultures of Phanerochaete chrysosporium was investigated to clarify the role of CDH in cellulose degradation. Decomposition of bacterial microcrystalline cellulose by CBH I was enhanced significantly in

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