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一般說明
The new Fumarase Assay Kit, MAK521, is now available! Fumarase (Fumarate Hydratase; EC 4.2.1.2) catalyzes the reversible hydration of fumarate to malate in the tricarboxylic acid (Krebs) cycle. The activity of fumarase is important as it produces reducing equivalents that drive the synthesis of ATP and maintains energy balance in aerobic metabolism. A deficiency of fumarase activity results in progressive encephalopathy.
應用
Fumarase Activity Colorimetric Assay Kit has been used to determine fumarase (FUM) activity.
適合性
适于测定不同生物样品内的延胡索酸酶活性，如细胞和组织培养上清液以及纯化所得的线粒体。
原則
The Fumarase Activity Colorimetric Assay Kit provides a simple and high-throughput ready procedure for measuring fumarase activity in a variety of tissues, cells, and isolated mitochondria. Fumarase activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of fumarase is the amount of enzyme that generates 1.0 mmole of NADH per minute at pH 9.5 at 37 °C.
仅试剂盒组分
产品编号
说明
- Fumarase Assay Buffer
- Fumarase Substrate
- Fumarase Enzyme Mix
- Fumarase Developer
- NADH Standard
- Fumarase Positive Control
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Skin Corr. 1B
儲存類別代碼
8A - Combustible corrosive hazardous materials
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