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一般說明
Mannitol dehydrogenase (MDH) is a NAD-dependent enzyme, obtained from several organisms like, Lactobacillus brevis, Leuconostoc mesenteroides, Absidia glauca and Rhodococcus. MDH obtained from L. mesenteroides exhibits similarity in sequence with the medium-chain dehydrogenase/reductase protein family. It also contains four identical subunits of about 38 kDa in size.
Mannitol dehydrogenase is a cytoplasmic enzyme whose primary role is the regulation of intracellular levels of the sugar alcohol mannitol in plants. It catalyzes the pyridine nucleotide dependent reduction of fructose to mannitol.
The molecular weight of mannitol dehydrogenase is 136 kDa.
應用
Can be used for the production of D-mannitol.
It has been used in a study to assess glucosylglycerol and glucosylglycerate as enzyme stabilizers.
This preparation is useful in the determination of mannitol in urine.
生化／生理作用
Mannitol dehydrogenase (MDH) helps to catalyze the pyridine nucleotide-dependent oxidation of D-mannitol to D-fructose. Mannitol dehydrogenase (MDH) obtained from L. mesenteroides is highly specific for the conversion fructose and mannitol.
單位定義
在 pH 5.3 和 30°C 且存在 NADH 的条件下，一单位本品每分钟将还原 1.0μmol D-果糖。如上定义的一单位酶相当于如 Lunn 等人所述的约 1.8 单位酶，并且在 pH 8.6 和 40°C 且存在 NAD 的条件下，一单位酶每分钟将氧化 1.0μmol D-甘露醇。
外觀
含缓冲盐、磷酸钾以及二硫苏糖醇的冻干粉
準備報告
部分纯化
分析報告
根据 Yamanaka, Meth. Enzymol., 41(B), 138 (1975) 所述方法进行分析。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves
Enzymatic production of D-mannitol with the Leuconostoc pseudomesenteroides mannitol dehydrogenase coupled to a coenzyme regeneration system
Parmentier S
Biocatalysis and Biotransformation, 23, 1-7 (2005)
Polyol metabolism in homopterans at high temperatures: accumulation of mannitol in aphids (Aphididae: Homoptera) and sorbitol in whiteflies (Aleyrodidae: Homoptera)
Hendrix D.L. , Salvucci, M.E.
Comparative Biochemistry and Physiology, 120, 487-494 (1998)
J Aarnikunnas et al.
Applied microbiology and biotechnology, 59(6), 665-671 (2002-09-13)
The N-terminal amino acid sequences of the intact protein and three tryptic peptides from a 41 kDa protein purified from a commercial mannitol dehydrogenase (MDH) enzyme preparation of Leuconostoc mesenteroides ATCC-9135 were determined. Oligonucleotides deduced from these peptide sequences were
P G Lunn et al.
Clinica chimica acta; international journal of clinical chemistry, 183(2), 163-170 (1989-08-15)
A need for a simple method for the determination of mannitol in urine has arisen because of the use of this monosaccharide in intestinal permeability tests. A rapid spectrophotometric assay for mannitol is presented based on the use of the
Purification and characterization of a novel mannitol dehydrogenase from Lactobacillus intermedius
Saha, B.
Biotechnology Progress, 20, 537-542 (2012)
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