M8433

Anti-phospho-c-Myc (pThr⁵⁸/pSer⁶²) antibody produced in rabbit

affinity isolated antibody, liquid

• MDL编号: MFCD02097158
• UNSPSC代码: 12352203

属性

• 生物来源: rabbit
• 偶联物: unconjugated
• 抗体形式: affinity isolated antibody
• 抗体产品类型: primary antibodies
• 克隆: polyclonal
• 表单: liquid
• 分子量: antigen ~64-67 kDa
• 种属反应性: rat, human, mouse
• 技术: immunohistochemistry: 10-20 μg/mL; immunoprecipitation (IP): 2-4 μg/mL; western blot: 0.5-4 μg/mL
• UniProt登记号: P01106
• 运输: wet ice
• 储存温度: −20°C
• 靶向翻译后修饰: phosphorylation (pThr58/pSer62)
• 基因信息: human ... MYC(4609) ; mouse ... Myc(17869) ; rat ... Myc(24577)

说明

一般描述

The transcription factor c-Myc is a short-lived nuclear phosphoprotein involved in cell proliferation, differentiation, and cancer. Expression of c-Myc is strictly controlled by mitogens and is required for cell cycle entry. c-Myc binds to DNA and activates transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro at Ser⁶² by MAP Kinase, and in vivo at both Thr⁵⁸ and Ser⁶² although the pathways and kinases acting in vivo have not been identified. Mutation of Thr⁵⁸ and Ser⁶² to Ala inhibits the ability of c-Myc to stimulate transcription. c-Myc, a ~45 kDa protein that migrates with an apparent molecular weight of ~64-67kDa in reducing SDS-PAGE, appears to regulate multiple aspects of growth control; c-Myc promotes cell proliferation in the presence of Cdk inhibitors and induces apoptosis upon overexpression in serum deprived cells.

特异性

Does not react with non-phosphorylated c-Myc.

免疫原

synthetic peptide corresponding to residues around phospho-Thr⁵⁸ and phospho-Ser⁶² of human c-Myc.

外形

In phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

制备说明

Purified by protein A and peptide affinity chromatography.

免责声明

For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable