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出版信息
J. Sambrook, E.F. Fritsch and T. Maniatis, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989, 1,626 pp., soft cover
制造商/商品名称
Cold Spring Harbor
重量
~10 lb
特点和优势
This, the most widely used handbook of molecular biology protocols, has been expanded to three volumes.
目录
Book 1
1. Plasmid Vectors. 2. Bacteriophage l Vectors. 3. Cosmid Vectors. 4. Single-stranded, Filamentous Bacteriophage Vectors. 5. Enzymes Used in Molecular Cloning. 6. Gel Electrophoresis of DNA. 7. Extraction, Purification, and Analysis of Messenger RNA from Eukaryotic Cells.
Book 2
8. Construction and Analysis of cDNA Libraries. 9. Analysis and Cloning of Eukaryotic Genomic DNA. 10. Preparation of Radiolabeled DNA and RNA Probes. 11. Synthetic Oligonucleotide Probes. 12. Screening Expression Libraries with Antibodies and Oligonucleotides. 13. DNA Sequencing. 14. In Vitro Amplification of DNA by the Polymerase Chain Reaction. 15. Site-directed Mutagenesis of Cloned DNA.
Book 3
16. Expression of Cloned Genes in Cultured Mammalian Cells. 17. Expression of Cloned Genes in Escherichia coli. 18. Detection and Analysis of Proteins Expressed from Cloned Genes.
Appendices
Appendix A: Bacterial Media, Antibiotics, and Bacterial Strains. Appendix B: Preparation of Reagents and Buffers Used in Molecular Cloning. Appendix C: Properties of Nucleic Acids. Appendix D: Codons and Amino Acids. Appendix E: Commonly Used Techniques in Molecular Cloning. Appendix F: Subcloning. Appendix G: List of Suppliers.
1. Plasmid Vectors. 2. Bacteriophage l Vectors. 3. Cosmid Vectors. 4. Single-stranded, Filamentous Bacteriophage Vectors. 5. Enzymes Used in Molecular Cloning. 6. Gel Electrophoresis of DNA. 7. Extraction, Purification, and Analysis of Messenger RNA from Eukaryotic Cells.
Book 2
8. Construction and Analysis of cDNA Libraries. 9. Analysis and Cloning of Eukaryotic Genomic DNA. 10. Preparation of Radiolabeled DNA and RNA Probes. 11. Synthetic Oligonucleotide Probes. 12. Screening Expression Libraries with Antibodies and Oligonucleotides. 13. DNA Sequencing. 14. In Vitro Amplification of DNA by the Polymerase Chain Reaction. 15. Site-directed Mutagenesis of Cloned DNA.
Book 3
16. Expression of Cloned Genes in Cultured Mammalian Cells. 17. Expression of Cloned Genes in Escherichia coli. 18. Detection and Analysis of Proteins Expressed from Cloned Genes.
Appendices
Appendix A: Bacterial Media, Antibiotics, and Bacterial Strains. Appendix B: Preparation of Reagents and Buffers Used in Molecular Cloning. Appendix C: Properties of Nucleic Acids. Appendix D: Codons and Amino Acids. Appendix E: Commonly Used Techniques in Molecular Cloning. Appendix F: Subcloning. Appendix G: List of Suppliers.
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