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Merck
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主要文件

L5390

Sigma-Aldrich

Lectin from Erythrina cristagalli (coral tree)

lyophilized powder

别名:

Erythrina cristagalli agglutinin, ECA

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About This Item

MDL编号:
UNSPSC代码:
12352202
NACRES:
NA.32

表单

lyophilized powder

质量水平

效能

<0.5 μg per mL agglutination activity

组成

Protein, ≥85% biuret

杂质

salt, free

储存温度

−20°C

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生化/生理作用

ECA is not blood group specific, but has an affinity for D-galactose and D-galactosides. It is reported to be mitogenic for human peripheral blood T lymphocytes.
Lectin or haemagglutinin is a sugar stereospecific binding protein or glycoprotein. It is capable of reversibly agglutinating cells. It is implicated in various cellular processes such as cell agglutination, homing of leukocytes, induction of mitosis in lymphocytes, interferon c (IFN c) and cytotoxicity. It is used as a blood typing reagent in immunohaematology. Lectin is also used as a reagent in testing erythrocyte polyagglutinable states. It is considered as a potential tool for analyzing cell surface architecture and related membrane functions.

制备说明

Purified by affinity chromatography.

分析说明

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of trypsinized human blood group O erythrocytes after 1 hr at 25 °C.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Human erythrocyte specific lectin from the seeds of Indian coral tree, Erythrina variegata Linn, var. orientali Linn, Merrill
Datta TK and Basu PS
Journal of Biosciences, 5(1), 25-30 (1983)
Purification, some properties of a D-galactose-binding leaf lectin from Erythrina indica and further characterization of seed lectin
Konozy EHE, et al.
Biochimie, 84(10), 1035-1043 (2002)

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