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Merck

L4144

Sigma-Aldrich

来自 菜豆的凝集素 (红芸豆)

Leucoagglutinin PHA-L, lyophilized powder, BioReagent, suitable for cell culture

别名:

PHA, 植物血球凝集素(菜豆)

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About This Item

EC號碼:
分類程式碼代碼:
12352202
NACRES:
NA.32

品質等級

產品線

BioReagent

形狀

lyophilized powder

效力

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.)
≤1.0 μg per mL leucoagglutination activity
≤5 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

技術

cell culture | mammalian: suitable

雜質

salt, free

溶解度

DPBS: 5 mg/mL, slightly hazy, colorless

適合性

leucocyte agglutination activity tested

儲存溫度

2-8°C

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一般說明

凝集素是糖类结合蛋白,由4个亚基组成,分子量为125 kDa。白细胞凝集素PHA-L是一种植物凝集素,通过中枢神经系统的神经元传递。

應用

凝集素,来自菜豆(红芸豆)已用于:
  • 用作处理细胞的阳性对照
  • 刺激细胞培养物用于淋巴细胞增殖分析
  • 刺激T细胞

生化/生理作用

凝集素可凝集细胞并沉淀多糖和糖蛋白。它与毒性过敏原和血凝素有关。凝集素呈现抗癌、抗人类免疫缺陷病毒(抗HIV)和抗微生物感染特性。凝集素可抑制黏膜萎缩、减轻2型糖尿病和肥胖症状、加强营养吸收并调节药物靶向。
PHA由两种分子种类组成,即具有低促有丝分裂活性和高红细胞凝集素活性的红细胞凝集素(PHA-E),和具有高促有丝分裂和白细胞凝集活性但具有非常低的红细胞凝集活性的白细胞凝集素(PHA-L)。
PHA-E不具有血型特异性,但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前,PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。

分析報告

凝集活性以 μg/ml 表示,并由 1 mg/ml 溶液的连续稀释液测定。该活性是在 25℃ 温育 1 小时后凝集人红细胞(2% 在磷酸盐缓冲盐水,pH6.8)或人白细胞(107/mL,在盐水中)的悬浮液的最低浓度。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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SARS-CoV-2 vaccination in rituximab-treated patients: B cells promote humoral immune responses in the presence of T-cell-mediated immunity.
Mrak, et al.
Annals of the Rheumatic Diseases (2021)
Patricia Kaaijk et al.
Scientific reports, 11(1), 13664-13664 (2021-07-03)
Mumps is nowadays re-emerging despite vaccination. The contribution of T cell immunity to protection against mumps has not been clearly defined. Previously, we described a set of 41 peptides that were eluted from human leukocyte antigen (HLA) class I molecules
Haifeng Huang et al.
Acta pharmaceutica Sinica. B, 10(7), 1321-1330 (2020-09-03)
JS001 (toripalimab) is a humanized IgG monoclonal antibody which strongly inhibits programmed cell death protein 1 (PD1). In this study, we used a different iodine isotype (nat/124/125I) to label JS001 probes to target the human PD1 (hPD1) antigen. In vitro
T follicular-like helper cells in the peripheral blood of patients with primary Sjogrens syndrome
Brokstad KA, et al.
Scandinavian Journal of Immunology, 88(2), e12679-e12679 (2018)
Christopher Lai-Hipp et al.
Transfusion, 51(2), 333-337 (2010-08-28)
Cellular substrate (CS) composed of phytohemagglutinin (PHA)-stimulated PBMNCs is required for isolating primary human immunodeficiency virus Type 1 from plasma (PHIV) during early acute infection. The transmitted founder PHIV is neutralization-sensitive and uses chemokine-receptor 5 to infect CD4-positive PBMNCs. Therefore

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