H4281

杂交瘤培养基

without antibiotics, antimycotics, L-glutamine, and phenol red, with sodium bicarbonate, HEPES, essential and non-essential amino acids, liquid, sterile-filtered, suitable for hybridoma

• 别名: Hybridoma Medium
• MDL號碼: MFCD03098239
• 分類程式碼代碼: 12352207
• NACRES: NA.75

属性

• 描述: endotoxin tested; for research or for further manufacturing use
• 無菌: sterile-filtered
• 形狀: liquid
• 技術: cell culture | hybridoma: suitable
• 成分: HEPES: yes; NaHCO₃: yes; L-glutamine: no; phenol red: no; glucose: 2.9 g/L
• 運輸包裝: ambient
• 儲存溫度: 2-8°C

说明

應用

Serum-free, low-protein medium formulated to support the growth of hybridomas used in the manufacture of monoclonal antibodies.

特點和優勢

Designed to support the rapid growth of cells to high densities and the maintenance of viable cells for extended culture periods. This formulation reduces raw material cost and the low protein level reduces cost associated with downstream processing. Purified components in the product reduce the variability sometimes encountered with biological materials leading to improved consistency of the medium performance. Cells can be successfully transferred from serum-supplemented cultures to this medium with little or no adaptation.

其他說明

This proprietary formulation includes inorganic salts, essential and non-essential amino acids, vitamins, sodium bicarbonate, HEPES, trace elements, fatty acids, and other organics. It contains low concentrations of recombinant human insulin, bovine serum albumin and human transferrin (source tested negative for HIV antibody and HbsAG). The medium does not contain L-glutamine, antibiotics, antimycotics, and phenol red.

注意

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time. When stored at 2-8°C in the dark, medium that has not been supplemented is stable until the expiration date on the label.

重構

Prior to use, medium should be warmed to 37°C and supplemented with 50 ml/L of 200 mM L-glutamine.

法律資訊

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable