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Merck
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H1038

Sigma-Aldrich

Hck human

recombinant, expressed in E. coli BL21, vial of ≥200 units

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About This Item

MDL號碼:
MFCD03792313
分類程式碼代碼:
12352204
NACRES:
NA.54

重組細胞

expressed in E. coli BL21

品質等級

200

形狀

solution

比活性

1-2 unit/μg protein

包裝

vial of ≥200 units

相關疾病

cancer (leukemia)

運輸包裝

dry ice

儲存溫度

−70°C

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應用

Hck is a protein-tyrosine kinase that is expressed in hemopoietic cells. It is used in leukemia research .

生化／生理作用

Hck is a member of the Src tyrosine kinase family. Hck has been shown to associate with and be activated by Bcr-Abl, which is a protein-tyrosine kinase that is expressed in chronic myelogenous leukemia .

單位定義

One unit will phosphorylate 1.0 picomole of Tyr15 of the peptide substrate pcdc2 6-20 per minute at 30 °C at pH 7.4.

外觀

Supplied as a solution in 50 mM Tris HCl, pH 7.4, 1 mM EDTA and 1 mM DTT

儲存類別代碼

10 - Combustible liquids

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J M Lionberger et al.
The Journal of biological chemistry, 275(24), 18581-18585 (2000-06-13)
Bcr-Abl is the constitutively active protein-tyrosine kinase expressed as a result of the Philadelphia translocation in chronic myelogenous leukemia. Bcr-Abl is coupled to many of the same signaling pathways normally regulated by hematopoietic cytokines. Recent work shows that Hck, a

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