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GE28-4082-61

HiTrap® MabSelect™ Xtra

Cytiva 28-4082-61, pack of 5 × 5 mL

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About This Item

UNSPSC代码:
23151816
NACRES:
NA.56

ligand

recombinant protein A (E. coli)

包装

pack of 5 × 5 mL

制造商/商品名称

Cytiva 28-4082-61

储存条件

(20% Ehtanol)
 (20% Ethanol)

参数

42 psi

柱床尺寸

16 mm × 25 mm

柱床体积

5 mL

色谱柱内径

16 mm

基质

highly cross-linked agarose

平均零件尺寸

75 (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-12

工作范围

3-10

容量

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

适用性

suitable for bioprocess medium

储存温度

2-8°C

相关类别

一般描述

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiTrap® MabSelect™ Xtra columns are prepacked with MabSelect™ Xtra, a BioProcess medium for capture of monoclonal antbodies from large sample volumes.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

应用

The increased capacity of the medium is well suited for purification of Mabs from high-level expression feedstock.

特点和优势

  • Increased dynamic binding capacity with smaller particle size and greater porosity than MabSelect™ medium.
  • MabSelect™ Xtra uses the same recombinant Protein A ligand as MabSelect™
  • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  • Prepacked HiTrap® columns for process development, screening of purification conditions, and small-scale purification of Mabs.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

HiTrap is a registered trademark of Cytiva
MabSelect is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

色谱柱填充和抗体亲和层析的制备

本页介绍了高效的色谱柱填充和抗体亲和层析的制备。

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

抗体亲和色谱的脱盐和缓冲液置换

实验室规模的脱盐是一种成熟、简单、快速的方法，可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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