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Merck

GE28-4064-15

Sigma-Aldrich

Tricorn 10/100 Column

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About This Item

分類程式碼代碼:
41121800
NACRES:
SB.52

技術

LPLC: suitable

一般說明

Tricorn 5 and 10 Coarse Filter Kit is used for large volume/repeated loading of clarified sample to reduce the risk of clogging in combination with capture media. Both filter and coarse filter kits include top and bottom adapters, bottom filters, and EPDM O-rings, five of each.
Tricorn columns include a glass tube, adapter unit, end cap, filter kit, two stop plugs, two fingertight connectors, two M6 connectors, adapter lock, and filter holder.

特點和優勢

Broad range of column dimensions suitable for many different purification needs.
Glass tubes coated with plastic film prevent harm if the column is dropped or operated outside its specification.
Locking ring prevents accidental compression of media bed.
Separate packing connector allows simple column packing for a broad range of media.
The modern integrated, snap-on design results in a column that is easy to assembly, operate, and maintain.
Tricorn empty columns are designed for high performance separations on small and medium scale. Pressure stability up to 100 bar and durable column material ensure reliable and reproducible operation as well as compatibility with the majority of the commercial lab media.

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The different stages of working with SOURCE™ (Cytiva) for high-resolution separation are discussed, from purification to polishing and scaling up

实验方案

Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.

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This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

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