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用途
sufficient for 24 reactions
制造商/商品名称
Cytiva 25-6603-24
存货情况
not available in Japan
储存温度
room temp
一般描述
GenomiPhi HY Ready-To-Go DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 40 to 60 μg yield from nanogram amounts of DNA sample.GenomiPhi HY Ready-To-Go DNA Amplification Kit is part of the Phi29 DNA polymerase family of products from Cytiva. It contains all of the components necessary for whole genome amplification by isothermal strand displacement amplification. The reaction mixture is provided as predispensed, single-dose, lyophilized cakes in either strips of 8 tubes, or 96-well plates.
Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample. Furthermore, amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity. The average product length is over 10 kb.
GenomiPhi HY Ready-To-Go DNA Amplification Kit procedure is quick, simple and does not require a thermocycler. A typical DNA yield of 40 to 60 µg DNA can be achieved in four hours from only 10 ng of genomic DNA input. The starting material for GenomiPhi reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used. Protocols for the amplification of DNA from blood lysates and dried blood spots are included in the manual.
GenomiPhi amplified DNA is suitable for various applications such as genotyping, cloning, sequencing, and DNA archival.
Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample. Furthermore, amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity. The average product length is over 10 kb.
GenomiPhi HY Ready-To-Go DNA Amplification Kit procedure is quick, simple and does not require a thermocycler. A typical DNA yield of 40 to 60 µg DNA can be achieved in four hours from only 10 ng of genomic DNA input. The starting material for GenomiPhi reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used. Protocols for the amplification of DNA from blood lysates and dried blood spots are included in the manual.
GenomiPhi amplified DNA is suitable for various applications such as genotyping, cloning, sequencing, and DNA archival.
应用
For midi-scale whole genome amplification by isothermal strand displacement amplification; typical downstream applications are genotyping (SNP, array CGH) and DNA archiving.
特点和优势
- Representative amplification of the whole genome.
- Preformulated, predispensed, single-dose, lyophilized cakes for rapid reaction set-up and greater reproducibility.
- Quick and simple automation-friendly protocol; no thermal cycler required.
- Shipping and storage at room temperature provides easy access and reduced carbon footprint.
- Yields high-quality DNA (e.g. for high-throughput genotyping and DNA archival).
储存及稳定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析说明
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律信息
GenomiPhi
On packaging and insert:
For use only as licensed by Qiagen GmbH. and Cytiva. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,576,204, and equivalent patents and patent applications in other countries.
On container:
Subject to proprietary rights of Cytiva. May not be re-sold or used except with the other components of this kit. See US patent numbers 5,576,204, and equivalent patents and patent applications in other countries.
On packaging and insert:
For use only as licensed by Qiagen GmbH. and Cytiva. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,576,204, and equivalent patents and patent applications in other countries.
On container:
Subject to proprietary rights of Cytiva. May not be re-sold or used except with the other components of this kit. See US patent numbers 5,576,204, and equivalent patents and patent applications in other countries.
GenomiPhi is a trademark of Cytiva
储存分类代码
13 - Non Combustible Solids
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