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包裝
pack of 5 × 1 mL
製造商/商標名
Cytiva 17-0920-03
參數
42 psi
床尺寸
7 mm × 25 mm
床體積
1 mL
柱內徑
7 mm
基質
highly cross-linked 6% agarose
樣品平均尺寸
34 μm
cleaning
2-14(Medium uncharged with metal ions.)
工作範圍
3-12
容量
≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
一般說明
應用
特點和優勢
- For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion.
- Conveniently charge with your metal of choice.
- Small bead size for high-performance purification.
- High binding capacity.
儲存和穩定性
分析報告
其他說明
法律資訊
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
商品
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
实验方案
This page shows how to purify histidine-tagged proteins using HiTrap® IMAC HP and HiTrap® IMAC FF from Cytiva.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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