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等级
Hybri-Max™
质量水平
无菌性
sterile-filtered
方案
≥99% (TLC)
表单
solution
技术
cell culture | hybridoma: suitable
杂质
endotoxin, tested
颜色
clear colorless
运输
dry ice
储存温度
−20°C
SMILES字符串
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
InChI key
ZDXPYRJPNDTMRX-VKHMYHEASA-N
基因信息
rat ... Ggt1(116568)
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一般描述
L-谷氨酰胺是一种必需氨基酸,作为细胞培养过程中主要的能量来源而成为培养基的重要组分。L-谷氨酰胺在干粉和冷冻溶液中非常稳定。然而,在液体培养基或储备溶液中,L-谷氨酰胺的降解速度相对更快。为获得最佳细胞性能,通常需要在使用培养基前补充L-谷氨酰胺。
应用
L-谷氨酰胺溶液已用作罗斯威尔公园纪念研究所(RPMI)-1640培养基的补充剂,以培养人白血病单核细胞系用于细胞内分析和脾细胞用于CD4+ T细胞的体外激活。它还用作杂交瘤培养基的补充剂,用于抗原结合和杂交瘤克隆的初级筛选。
制备说明
使用细胞培养级水进行制备。
法律信息
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
应用
储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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Chromatograms
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