LB253

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: human blood
• 包装: tube of 5 μg 98062330-DNA-5UG; pkg of vial of cells 98062330-1VL
• 生长模式: Suspension
• 核型: Not specified
• 形态学: Lymphoblastoid
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human lymphocytes, Xeroderma pigmentosum group C, EBV-transformed

细胞系描述

Derived from mononuclear cells from the peripheral blood of a 67 year old male. The patient was diagnosed with Xeroderma pigmentosum group C and showed spontaneous regression of melanomas. A normal level of natural killer (NK) cell activity and number, despite marked lymphopenia, was reported. The original sample was used in UV irradiation studies where UV-sensitivity of lymphocytes and fibroblasts was detected. In addition, a defect in excision repair was reported.

应用

DNA repair deficiency studies, hprt mutant analysis

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9,13
D5S818: 11,12
D7S820: 8,12
THO1: 6,9.3
TPOX: 8
vWA: 17,19

培养基

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

传代培养常规

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml 5%

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.