LB205

98062329, human blood, Lymphoblastoid

• 分類程式碼代碼: 41106514

属性

• product name: LB205, 98062329
• 生物源: human blood
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Lymphoblastoid
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human lymphocytes, Xeroderma pigmentosum variant, EBV-transformed

細胞系描述

Derived from mononuclear cells from the peripheral blood of a 62 year old male. The patient was diagnosed with Xeroderma pigmentosum variant, illustrating that the disease is heterogeneous. T cells from the original sample have been used for UV irradiation studies and hprt mutant frequency. The cell line has been established by transformation with EBV. Fibroblasts from the same individual are designated XP11 BR.

應用

DNA repair deficiency studies, hprt mutation analysis

培養基

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

例行更新培養

Maintain cultures between 3-9 x 100,000 cells/ml dilute every 1-2 days to 3 x 100,000 cells/ml 5%

其他說明

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