MFE-280

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 98050131, human endometrium, Epithelial

• 别名: MFE280 Cells
• UNSPSC代码: 41106514

属性

• 生物来源: human endometrium
• 包装: tube of 5 μg 98050131-DNA-5UG; pkg of vial of cells 98050131-1VL
• 生长模式: Adherent
• 核型: Near diploid
• 形态学: Epithelial
• 产品: Placental protein 14
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human caucasian endometrial adenocarcinoma

细胞系描述

MFE-280 was derived from a recurrent, poorly differentiated endometrial carcinoma from a 78 year old patient. Initially, cells were grown as aggregates in suspension for one year, after which they were transferred to monolayer culture. The karyotype analysis revealed near-diploidy with a complex heterogeneous aberration pattern. MFE-280 were found to be positive for cytokines 7, 8, 18 and 19 as well as for vimentin. In addition, the cells express the placental protein 14, PP14. They have been shown to be tumorigenic in nude mice.

应用

Tumour biology studies

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 9,12
D13S317: 10,12
D16S539: 12
D5S818: 11,12
D7S820: 10
THO1: 7
TPOX: 9,10
vWA: 17

培养基

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding 2-4 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.