A373-C6

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: human skin
• 包装: tube of 5 μg 97011321-DNA-5UG; pkg of vial of cells 97011321-1VL
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Epithelial-like
• 产品: Not specified
• 受体: IL-1 receptor
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human malignant melanoma IL-1 sensitive

细胞系描述

The cell line A375-C6 has been shown to be sensitive to the IL-1 mediated anti-proliferative effect. It has been derived from the parental line A375 (Sigma Catalogue number. 88113005) by limiting dilution together with its twin clone A375-C5 (Sigma Catalogue number. 97011320) which is IL-1 unresponsive. A375-C6 could be useful for a growth inhibition assay for IL-1. The cells have been eradicated from mycoplasma at ECACC.

应用

IL-1 growth inhibition assay, IL-1 receptor analysis

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 8
TPOX: 8,10
vWA: 16,17

培养基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 1-3 x 10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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