EMT6/VRP/R

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: mouse unknown/unspecified
• 包装: tube of 5 μg 96042319-DNA-5UG; pkg of vial of cells 96042319-1VL
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Epithelial-like
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse mammary, drug-resistant

细胞系描述

The drug-resistant line EMT6/VRP/R was established from the parent cell line EMT6/P (Sigma Catalogue number. 96042344) by growth in verapamil (see reference).

应用

Drug-resistance studies

培养基

MEM (EBSS) +2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 75 μg/ml verpamil + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂;37ºC. It is recommended to resuscitate cells in drug-free medium. Add drug after growth is established.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.