SK-N-BE(2)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95011815, human nerve, Epitheilioid & neuroblastoid

• UNSPSC代码: 41106514

属性

• 生物来源: human nerve
• 包装: tube of 5 μg 95011815-DNA-5UG; pkg of vial of cells 95011815-1VL
• 生长模式: Adherent
• 核型: Modal no. 44
• 形态学: Epitheilioid & neuroblastoid
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: metastasis
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human Caucasian neuroblastoma

细胞系描述

Established from a bone marrow biopsy of a 22-month-old male with disseminated neuroblastoma in 1972. The morphology varies with sub-population from cells with long processes through small, flattened, epithelioid cells. Dense cultures tend to form loosely adherent aggregates. They are multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine.

应用

Chemotherapy development, chromosome studies, drug resistance, differentiation studies.

培养基

EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham′s F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:10 to 1:25 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.